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Triple combination therapy alleviates colorectal cancer immunosuppression by targeting immune cells and cytokine networks. (A-E) The ratio of CD4 + CD25 + Foxp3 + Tregs among CD4 + cells in the tumor, spleen, TDLNs, and NTDLNs (n=5). (F-J) Percentage of Tim3 + cells among CD8 + T cells in the tumor, spleen, TDLNs, and NTDLNs(n=5). (K-O) Serum circulating <t>cytokines</t> were quantified from the serum samples, including IL-2, IL-10, TNF-α, TGF-β, IL-6 with 5 samples each in an experimental group. (All data are presented as mean ± SD and were analyzed using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = no significant difference, P > 0.05.).
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Triple combination therapy alleviates colorectal cancer immunosuppression by targeting immune cells and cytokine networks. (A-E) The ratio of CD4 + CD25 + Foxp3 + Tregs among CD4 + cells in the tumor, spleen, TDLNs, and NTDLNs (n=5). (F-J) Percentage of Tim3 + cells among CD8 + T cells in the tumor, spleen, TDLNs, and NTDLNs(n=5). (K-O) Serum circulating cytokines were quantified from the serum samples, including IL-2, IL-10, TNF-α, TGF-β, IL-6 with 5 samples each in an experimental group. (All data are presented as mean ± SD and were analyzed using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = no significant difference, P > 0.05.).

Journal: Translational Oncology

Article Title: Combination of APS, HMGN1, and anti-TNFR2 antibody remodels the tumor immune microenvironment to enhance antitumor immunity in colorectal cancer

doi: 10.1016/j.tranon.2026.102814

Figure Lengend Snippet: Triple combination therapy alleviates colorectal cancer immunosuppression by targeting immune cells and cytokine networks. (A-E) The ratio of CD4 + CD25 + Foxp3 + Tregs among CD4 + cells in the tumor, spleen, TDLNs, and NTDLNs (n=5). (F-J) Percentage of Tim3 + cells among CD8 + T cells in the tumor, spleen, TDLNs, and NTDLNs(n=5). (K-O) Serum circulating cytokines were quantified from the serum samples, including IL-2, IL-10, TNF-α, TGF-β, IL-6 with 5 samples each in an experimental group. (All data are presented as mean ± SD and were analyzed using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = no significant difference, P > 0.05.).

Article Snippet: Serum levels of certain cytokines (IL-2(Cat. No. GEM0038), IL-6(Cat. No. GEM0001), IL-10(Cat. No. GEM0003), TNF-α (Cat. No. GEM0004), TGF-β (Cat. No. GEM0051) were detected by ELISA kits (Servicebio, Wuhan, China) according to the instructions from the manufacturer.

Techniques: